Effect of alloxan diabetes on a Ca2+-activated proteinase in rat skeletal muscle
- 1 March 1983
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 244 (3), C175-C181
- https://doi.org/10.1152/ajpcell.1983.244.3.c175
Abstract
The effect of making rats diabetic by alloxan injection on activity of the muscle Ca2+-activated proteinase (CAF) was investigated. Groups of four to seven control or alloxan-injected rats were killed 10 min (0 day) and 10, 17, and 24 days after a second alloxan injection. The second alloxan injection was given 3 days after the first. CAF activity was assayed in fractions precipitated between 0 and 45% ammonium sulfate saturation (P0-45 crude CAF fractions) that had been prepared so as to remove the protein inhibitor of CAF. Gel permeation, followed by DEAE-cellulose chromatography of pooled P0-45 crude CAF fractions from each time-treatment group, demonstrated that the assays used in this study were specific for CAF activity. Muscle CAF activity was up to 50% higher in alloxan-injected rats than in control rats, regardless of whether activity was expressed per gram sarcoplasmic protein, per gram contractile protein, or per gram skeletal muscle fresh weight. Alloxan injection diminished rate of muscle mass accumulation but did not change the proportion of sarcoplasmic or contractile protein in skeletal muscle. Hence, alloxan injection decreased the rate of contractile protein deposition. The elevation of muscle CAF activity in alloxan-injected rats is consistent with the proposed role of CAF in initiating metabolic turnover of myofibrillar proteins but does not prove this role nor exclude participation of other proteinases.This publication has 27 references indexed in Scilit:
- Immunocytochemical localization of a calcium-activated protease in skeletal muscle cellsExperimental Cell Research, 1981
- Canine cardiac calcium‐dependent proteases: Resolution of two forms with different requirements for calciumFEBS Letters, 1980
- Neutral-alkaline proteases and protein degradation in rat heartJournal of Molecular and Cellular Cardiology, 1979
- MORPHOLOGIC ASPECTS OF MUSCLE BREAKDOWN AND LYSOSOMAL ACTIVATION *Annals of the New York Academy of Sciences, 1979
- Insulin Effect on Proteolytic Activities in Rat Skeletal MuscleHormone and Metabolic Research, 1978
- Early changes in the ultrastructure of denervated rat skeletal muscleExperimental Neurology, 1977
- EFFECT OF POSTMORTEM STORAGE AND CALCIUM ACTIVATED FACTOR ON THE MYOFIBRILLAR PROTEINS OF BOVINE SKELETAL MUSCLEJournal of Food Science, 1977
- A calcium(2+) ion-activated protease possibly involved in myofibrillar protein turnover. Partial characterization of the purified enzymeBiochemistry, 1976
- A Ca2+ion-activated protease possibly involved in myofibrillar protein turnover. Purification from porcine muscleBiochemistry, 1976
- Age‐Associated Changes in Bovine Muscle Connective Tissue. I. Rate of Hydrolysis by CollagenaseaJournal of Food Science, 1964