Thy‐1 positive tingible body macrophages (TBM) in mouse lymph nodes
- 1 December 1988
- journal article
- research article
- Published by Wiley in The Anatomical Record
- Vol. 222 (4), 380-390
- https://doi.org/10.1002/ar.1092220410
Abstract
This study was prompted by the observation that cells in mouse lymph nodes (LN) with cytological characteristics of tingible body macrophages (TBM) appeared to be Thy-1 positive. The objective of this study was to determine if these large cells were TBM and to conclusively demonstrate their reactivity for Thy-1. The cells were studied using monoclonal antibodies (MoAb) against Thy-1 and macrophage markers including F4/80 and Ia antigens at both light microscopic (LM) and electron microscopic (EM) levels. Immunocytochemical reactivity by TBM for Thy-1 antigen specific MoAb was demonstrated by LM in both in situ and in vitro LN preparations. Furthermore, ultrastructural examination of these germinal center cells in situ demonstrated that the Thy-1 reactivity visualized at the LM level was associated with ribosomes and endoplasmic reticulum as well as their plasma membrane. Similarly, these cells expressed intracytoplasmic and membrane reactivity for Ia antigens and also for the macrophage specific antigen F4/80. This indicates that the reactivity is due to active synthesis of the Thy-1 antigen and not attributable to reactivity of any phagocytosed Thy-1 positive cells. As defined by their germinal center location and morphological characteristics, these Thy-1 reactive macrophages were identified as TBM. Germinal center TBM thus represent a unique, vigorously phagocytic subset of mature macrophages which express both macrophage and thymocyte markers.This publication has 18 references indexed in Scilit:
- Antigenic phenotyping of isolated and in situ rodent follicular dendritic cells (FDC) with emphasis on the ultrastructural demonstration of Ia antigensThe Anatomical Record, 1986
- The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Relationship between macrophages, Langerhans cells, reticular cells, and dendritic cells in lymphoid and hematopoietic organs.The Journal of Experimental Medicine, 1983
- Expression of the macrophage-specific antigen F4/80 during differentiation of mouse bone marrow cells in culture.The Journal of Experimental Medicine, 1981
- F4/80, a monoclonal antibody directed specifically against the mouse macrophageEuropean Journal of Immunology, 1981
- The Maintenance and Regulation of the Humoral Immune Response: Persisting Antigen and the Role of Follicular Antigen‐Binding Dendritic Cells as Accessory CellsImmunological Reviews, 1980
- Non-Lymphatic Cells in the Lymph Node Cortex of the MousePathology - Research and Practice, 1980
- Studies of Ia Antigens on Murine Peritoneal MacrophagesScandinavian Journal of Immunology, 1976
- NONDROPLET ULTRASTRUCTURAL DEMONSTRATION OF CYTOCHROME OXIDASE ACTIVITY WITH A POLYMERIZING OSMIOPHILIC REAGENT, DIAMINOBENZIDINE (DAB)The Journal of cell biology, 1968
- ELECTRON MICROSCOPE OBSERVATIONS ON TINGIBLE BODY MACROPHAGES IN MOUSE SPLEENThe Journal of cell biology, 1963
- Specificity of Isoantisera against Leukæmic and Thymic LymphocytesNature, 1963