Voltammetric studies of damages in DNA by mutagenic and carcinogenic alkylating chemicals
- 1 June 1985
- journal article
- research article
- Published by Taylor & Francis in Toxicological & Environmental Chemistry
- Vol. 10 (2), 83-101
- https://doi.org/10.1080/02772248509357095
Abstract
Voltammetry provides new insights into the effects exerted in vitro by methylation of native DNA. Applying single sweep voltammetry at a stationary mercury electrode the successive steps of the destabilization of alkylated DNA are investigated. The methylation of the nucleic acids is manifested by a specific electrochemical response, due to the 7‐methylguanine, a major product of the methylation of DNA. Short time effects of the methylation include the labilization of 4 to 5 base pairs per methylated guanine base. Furthermore, uncommon protonation properties of the base 7‐methylguanine‐cytosine have been detected. Long term effects of the methylation are ultimately spontaneous hydrolytic strand breaks induced by the prior depurination connected with the release of the 7‐methylguanine from the methylated DNA. A half time t1/2 of 102 h for the depurination at 37°C has been determined. The depurination and the subsequent strand breaks alter the hydrodynamic properties of the damaged DNA, an effect which can be sensitively followed with voltammetry via the resulting changes in the diffusion coefficient of the damaged biopolymer.Keywords
This publication has 23 references indexed in Scilit:
- A New Electrochemical Approach for thein VitroInvestigation of Damage in Native DNA by Small γ-radiation DosesInternational Journal of Radiation Biology, 1982
- Prevention of contamination and other accuracy risks in voltammetric trace metal analysis of natural watersAnalytical and Bioanalytical Chemistry, 1980
- Overview of some aspects of occurrence, formation and analysis of nitrosaminesScience of The Total Environment, 1979
- 241 - Voltammetric studies on the bioelectrochemical behaviour of ultrasound sonicated and γ-irradiated native DNABioelectrochemistry and Bioenergetics, 1978
- Electrochemical behaviour of natural and biosynthetic polynucleotides at the mercury electrode V. stages of deconformation of native DNA at charged interfacesBioelectrochemistry and Bioenergetics, 1976
- Removal of minor methylation products 7-methyl adenine and 3-methylguanine from DNA ofescherichia coli treated with dimethyl sulphateChemico-Biological Interactions, 1976
- An Analysis of the Structure of tRNAAnnual Review of Biophysics and Bioengineering, 1975
- The electrochemical behaviour of DNA at electrically charged interfacesEuropean Biophysics Journal, 1974
- Alterations in the structure of deoxyribonucleic acid on chemical methylationBiochemistry, 1971
- A Study of Chemically Methylated Deoxyribonucleic AcidEuropean Journal of Biochemistry, 1971