The inactivation of oestrone

Abstract

Estrone was readily inactivated by H2O2 in alkaline soln. but not in neutral soln.; the methyl ether of estrone was not inactivated by H2O2 in alkaline soln. Estrone, estradiol, and diethylstilboestrol were inactivated by incubation with tyrosinase; a boiled tyrosinase extract had no action. Incubation with catechol oxidase or a combination of catechol oxidase plus catechol did not produce any appreciable in- activation. Complete inactivatioa resulted from the addition of H2O2, but could be prevented by the simultaneous addition of catalase; the addition of catalase did not prevent the inactivation by tyrosinase.