Fast Neutron and X-Ray Induced Single Strand DNA Breaks in Cultured Mammalian Cells

Abstract

The relative biologic effectiveness (RBE) of fast neutrons in the production of single strand DNA breaks is 1.6 as compared to that of 250 kVp x-rays. Monolayers of mouse L-929 cells were treated with dinotrophenol during irradiation to prevent the DNA strands from rejoining; the extent of DNA damage was measured by the alkaline sucrose sedimentation method. The RBE for DNA damage is essentially the same as the RBE measured by cell survival methods.