CORRELATION OF TIME-TEMPERATURE INDICATOR RESPONSE WITH MICROBIAL GROWTH IN PASTEURIZED MILK

Abstract
Commercially obtained pasteurized whole milk was stored at three constant temperatures (0°C, 5°C, and 10°C), and one variable temperature condition (cyclic exposure of 0°C for 14 days and 10°C for 2 days). Daily analyses were conducted to enumerate the growth of total bacteria, coliforms, psychrotrophs, and spore forming organisms in samples from each storage treatment. Microbial growth was correlated with the response of the I-POINT and LifeLine full-history time-temperature indicators. Response of the I-POINT model 2140 was strongly related to germination of the psychrotrophic bacteria, and significant correlations (r > 0.95) were found between total count enumeration and the LifeLine model 57 indicator.