Starvation or diabetes decreases the content but not the mRNA of 6‐phosphofructo‐2‐kinase in rat liver
- 25 January 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 227 (2), 136-140
- https://doi.org/10.1016/0014-5793(88)80884-6
Abstract
In rat liver, the activity of 6-phosphofructo-2-kinase (PFK-2) decreases upon starvation and in diabetes. Cyclic AMP-dependent phosphorylation of the enzyme is not sufficient to account for this decrease. PFK-2 content was therefore measured by immunotitration and relative PFK-2 mRNA levels were determined by hybridization with cDNA probes. The data are compatible with a posttranscriptional mechanism of regulation that involves decreased translational efficiency of PFK-2 mRNA and(or) increased turnover of the PFK-2 protein.Keywords
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