Roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection.
Open Access
- 1 November 1993
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 123 (3), 681-689
- https://doi.org/10.1083/jcb.123.3.681
Abstract
Previous studies suggest that kinesin heavy chain (KHC) is associated with ER-derived membranes that accumulate in the mitotic apparatus in cells of early sea urchin embryos (Wright, B. D., J. H. Henson, K. P. Wedaman, P. J. Willy, J. N. Morand, and J. M. Scholey. 1991. J. Cell Biol. 113:817-833). Here, we report that the microinjection of KHC-specific antibodies into these cells has no effect on mitosis or ER membrane organization, even though one such antibody, SUK4, blocks kinesin-driven motility in vitro and in mammalian cells. Microinjected SUK4 was localized to early mitotic figures, suggesting that it is able to access kinesin in spindles. In contrast to KHC-specific antibodies, two antibodies that react with kinesin-like proteins (KLPs), namely CHO1 and HD, disrupted mitosis and prevented subsequent cell division. CHO1 is thought to exert this effect by blocking the activity of a 110-kD KLP. The relevant target of HD, which was raised against the KHC motor domain, is unknown; HD may disrupt mitosis by interfering with an essential spindle KLP but not with KHC itself, as preabsorption of HD with KHC did not alter its ability to block mitosis. These data indicate that some KLPs have essential mitotic functions in early sea urchin embryos but KHC itself does not.Keywords
This publication has 26 references indexed in Scilit:
- A plus-end-directed motor enzyme that moves antiparallel microtubules in vitro localizes to the interzone of mitotic spindlesNature, 1992
- Motor proteins in cell divisionTrends in Cell Biology, 1991
- Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm [published erratum appears in J Cell Biol 1991 Aug;114(4):following 863]The Journal of cell biology, 1991
- Kinesin-related gene unc-104 is required for axonal transport of synaptic vesicles in C. elegansCell, 1991
- A monoclonal antibody to a mitotic microtubule-associated protein blocks mitotic progression.The Journal of cell biology, 1990
- Potential Roles of Microtubule Associated Motor Molecules in Cell DivisionAnnals of the New York Academy of Sciences, 1990
- Distribution of a matrix component of the midbody during the cell cycle in Chinese hamster ovary cellsThe Journal of cell biology, 1988
- Rapid visual detection of sperm-egg fusion using the DNA-specific fluorochrome Hoechst 33342Developmental Biology, 1986
- The role of spindle microtubules in the timing of the cell cycle in echinoderm eggsJournal of Experimental Zoology, 1986
- V-2 MicromanipulationCell Structure and Function, 1984