Single-step purification and h.p.l.c. analysis of glutathione transferase 8–8 in rat tissues
- 15 June 1989
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 260 (3), 785-788
- https://doi.org/10.1042/bj2600785
Abstract
GSSG selectively elutes two GSH transferases from a mixture of rat GSH transferases bound to a GSH-agarose affinity matrix. One is a form of GSH transferase 1-1 and the other is shown to be GSH transferase 8-8. By using tissues that lack this form of GSH transferase 1-1 (e.g. lung), GSH transferase 8-8 may thus be purified from cytosol in a single step. Quantitative analysis of the tissue distribution of GSH transferase 8-8 was obtained by h.p.l.c.This publication has 17 references indexed in Scilit:
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