Bulk Isolation in Nonaqueous Media of Nuclei from Lyophilized Cells

Abstract

Intact lyophilized nuclei are obtainable from a variety of tissues, either in situ or in culture, by freezing at -156°C, drying at -25°C, and mechanical disassociation in glycerol at 2°C. Centrifugal separation of nuclei is accomplished in an 85 : 15 by volume mixture of glycerol and 3-chloro-1,2 propanediol at 2°C. The method gives homogeneous nuclear preparations in high yield with preservation of labile and water-soluble constituents.