Membrane inserted APP fragments containing the βA4 sequence of Alzheimer's disease do not aggregate
- 31 August 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 309 (1), 20-24
- https://doi.org/10.1016/0014-5793(92)80730-5
Abstract
Previously we have shown that the COOH-terminal fragment (A4CT) of the Alzheimer amyloid protein precursor (APP), which at the NH2-terminus carries the sequence of the amyloid βA4 protein, forms highly insoluble aggregates [EMBO J. (1988) 7, 949–957]. Here we report that aggregation is prevented if A4CT is expressed in vitro with a signal sequence at the NH2-terminus (SPA4CT) under conditions which allow membrane insertion. Aggregates from SPA4CT are obtained after removal of membranes by chloroform/methanol extraction or heating.Keywords
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