Cloning and sequencing of four structural genes for the Na+‐translocating NADH‐ubiquinone oxidoreductase of Vibrio alginolyticus
Open Access
- 19 December 1994
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 356 (2-3), 333-338
- https://doi.org/10.1016/0014-5793(94)01275-x
Abstract
Oligonucleotide probes based on the N‐terminal amino acid sequences of the NqrA and NqrC subunits were used to clone genes for the Na+‐dependent NADH‐ubiquinone oxidoreductase complex from Vibrio alginolyticus. Four consecutive ORFs were identified encoding subunit proteins of 48.6, 46.8, 27.7 and 22.6 kDa, respectively (NqrA—D). A further ORF, showing 71% homology to the BolA protein of Escherichia coli, was located upstream. From sequence comparisons, we conclude that the Na+‐dependent NADH‐ubiquinone oxidoreductase complex of V. alginolyticus is clearly distinct from the corresponding H+‐dependent enzymes of both prokaryotes and eukaryotes.Keywords
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