Heparinized Human Umbilical Vein Grafts

Abstract

Tanned human umbilical vein grafts (Dardik Biograft and Meadow) were heparinized with tritium-labeled heparin. Two methods of heparinization were undertaken: incubation with heparin followed by ethyl alcohol 50% treatment, and incubation with heparin without alcohol. Grafts were placed in the carotid arteries of sheep and removed 10 and 90 days later. Heparin was lost in contact with blood but the alcohol treatment delayed heparin desorption significantly. [Heparin bonding to foreign surfaces is a common method used in attempts to prevent thrombosis.].