Effect of Inhibitors of Nucleic Acid and Protein Synthesis on Light-induced Synchronous Conidiation in Botrytis cinerea

Abstract

The effect of 5-fluoro-2-deoxyuridine (FdUrd), 6-azauridine, miracil D, 5-fluorouracil, cycloheximide, actinomycin D and puromycin on light-induced synchronous conidiation in B. cinerea Pers. ex Fr. is described. FdUrd, 6-azauridine, miracil D, 5-fluorouracil and cycloheximide inhibited photoinduced conidiation at the appropriate concentrations. Suppression of conidiation was greater when these inhibitors were added just before, rather than after, the photoinduction period. Actinomycin D and puromycin, both at 10-4 to 10-6 M, were ineffective. Incorporation of labeled precursors into nucleic acids and protein in the presence of FdUrd, miracil D, 5-fluorouracil and cycloheximide was greatly suppressed. The results suggest the involvement of nucleic acids and proteins in light-induced conidiation in B. cinerea.