L-Arginine, nitric oxide, and intestinal secretion: studies in rat jejunum in vivo.
Open Access
- 1 October 1996
- Vol. 39 (4), 539-544
- https://doi.org/10.1136/gut.39.4.539
Abstract
BACKGROUND: L-Arginine has been shown to induce fluid secretion in human jejunum. Nitric oxide, a derivative of L-arginine is thought to have an important role as an intestinal secretagogue. AIM: To determine the effect of L-arginine and the nitric oxide synthase inhibitor, nitro L-arginine methyl ester (L-NAME), on fluid and electrolyte movement in rat jejunum. METHODS: A 25 cm segment of rat jejunum was perfused in situ with iso-osmotic solutions containing either (1) saline, (2) D-arginine 20, (3) L-arginine 20, (4) L-NAME 0.1, 1, or 20 mmol/l, or (5) a combination of L-arginine 20 and L-NAME 0.1, 1, or 20 mmol/l. In further groups the effect of a subcutaneous injection of L-NAME 100 mg/kg was examined in rats pretreated with either D-or L-arginine 500 mg/kg. RESULTS: L-Arginine, unlike D-arginine, induced fluid secretion despite being better absorbed (mean -7.3 v 17.0 microliters/min/g; p < 0.01). L-NAME at 0.1 mmol/l had no effect on basal fluid movement but reversed L-arginine induced secretion (7.8; p < 0.05). L-NAME at 1 and 20 mmol/l induced fluid secretion (-15.4 and -28.4, respectively), which was enhanced by the addition of L-arginine (-30.0 and -41.0, respectively; both p < 0.05). A subcutaneous injection of L-NAME resulted in marked fluid secretion (-39.9) and histological evidence of intestinal ischaemia. These changes were attenuated or reversed by pretreatment with subcutaneous L- but not D-arginine. CONCLUSIONS: L-arginine induces intestinal fluid secretion through production of nitric oxide. There is a delicate balance between the effect of nitric oxide as a secretagogue and its effect on maintaining blood flow and thus preventing intestinal ischaemia.Keywords
This publication has 33 references indexed in Scilit:
- Nitric oxide-donating compounds stimulate electrolyte transport in the guinea pig intestine in vitroLife Sciences, 1993
- Direct Evidence for Nitric Oxide Stimulation of Electrolyte Secretion in the Rat ColonFree Radical Research Communications, 1993
- Endogenous nitric oxide: physiology, pathology and clinical relevanceEuropean Journal of Clinical Investigation, 1991
- Hydrogen peroxide stimulates rat colonic prostaglandin production and alters electrolyte transport.JCI Insight, 1990
- Immune system control of rat and rabbit colonic electrolyte transport. Role of prostaglandins and enteric nervous system.JCI Insight, 1989
- Efficacy of Oral Rehydration Solutions in a Rat Model of Secretory DiarrheaJournal of Pediatric Gastroenterology and Nutrition, 1987
- Jejunal water and electrolyte secretion induced by L-arginine in man.Gut, 1981
- Source of net water and electrolyte loss following intestinal ischaemiaZeitschrift für Die Gesamte Experimentelle Medizin, 1980
- The effect of amino acids and dipeptides on sodium and water absorption in manGut, 1973
- The recovery of function and microcirculation in small intestinal loops following ischaemiaGut, 1972