Calcium ion dependency of ethylene production in segments of primary roots of Zea mays
- 1 August 1986
- journal article
- research article
- Published by Wiley in Physiologia Plantarum
- Vol. 67 (4), 570-575
- https://doi.org/10.1111/j.1399-3054.1986.tb05057.x
Abstract
We investigated the effect of Ca2+ on ethylene production in 2-cm long apical segments from primary roots of corn (Zea mays L., B73 .times. Missouri 17) seedlings. The seedlings were raised under different conditions of Ca2+ availability. Low-Ca and high-Ca seedlings were raised by soaking the grains and watering the seedlings with distilled water or 10 mM CaCl2, respectively. Segments from high-Ca roots produced more than twice as much ethylene as segments from low-Ca roots. Indoleacetic acid (IAA; 1 .mu.M) enhanced ethylene production in segments from both low-Ca and high-Ca roots but auxin-induced promotion of ethylene production was consistently higher in segments from high-Ca roots. Addition of 1-aminocyclopropane-1-carboxylic acid (ACC) to root segments from low-Ca seedlings doubled total ethylene production and the rate of production remained fairly constant during a 24 h period of monitoring. In segments from high-Ca seedlings ACC also increased total ethylene production but most of the ethylene was produced within the first 6 h. The data suggest that Ca2+ enhances the conversions of ACC to ethylene. The terminal 2 mm of the root tip were found to be especially important to ethylene biosynthesis by apical segments and, experiments using 45Ca2+ as tracer indicated that the apical 2 mm of the root is the region of strongest Ca2+ accumulation. Other cations such as Mn2+, Mg2+ and K+ could largely substitute for Ca2+. The significance of these findings is discussed with respect to recent evidence for gravity-induced Ca2+ redistribution and its relationship to the establishment of asymmetric growth during gravitropic curvature.Keywords
This publication has 25 references indexed in Scilit:
- Analysis of growth patterns during gravitropic curvature in roots ofZea mays by use of a computer-based video digitizerJournal of Plant Growth Regulation, 1986
- Calcium Dependence of Rapid Auxin Action in Maize RootsPlant Physiology, 1986
- Cellular and subcellular localization of calcium in gravistimulated corn rootsProtoplasma, 1985
- Effects of Ethephon, 1-Aminocyclopropane-1-carboxylic Acid, and Inhibitors of Ethylene Synthesis on the Gravitropically Induced Movement of Mimosa pudica PulvinusPlant Physiology, 1985
- Role of Calcium in the Polar Secretion of Indoleacetic AcidPlant Physiology, 1984
- Calcium effects on ethylene and ethane production and 1‐aminocyclopropane‐1‐carboxylic acid content in potato disksPhysiologia Plantarum, 1984
- Calcium antagonists and calmodulin inhibitors block cytokinin-induced bud formation in FunariaDevelopmental Biology, 1983
- Influence of Calcium and Magnesium on Ethylene Production by Apple Tissue SlicesPlant Physiology, 1982
- Rapidly Induced Wound Ethylene from Excised Segments of Etiolated Pisum sativum L., cv. AlaskaPlant Physiology, 1978
- Stimulation of Ethylene Production in the Mung Bean Hypocotyls by Cupric Ion, Calcium Ion, and KinetinPlant Physiology, 1976