Expression and assembly of functional bacterial luciferase in plants
- 1 January 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (1), 131-135
- https://doi.org/10.1073/pnas.84.1.131
Abstract
The luxA and luxB structural genes of Vibrio harveyi luciferase [alkanal, reduced FMN:oxygen oxidoreducatase (1-hydroxylating, luminescing), EC 1.14.14.3] were introduced into a plant expression vector and transferred into tobacco and carrot cells by Agrobacterium-mediated or direct DNA transformation. Simultaneous expression of the luxA and luxB genes was monitored by protein immunoblot analysis. Luciferase-mediated light emission provided evidence for the assembly of the two protein subunits into a functional dimeric enzyme in plant protoplast, in transformed calli, and in leaves of transformed plants. Bacterial luciferase may provide a useful marker-gene system for the quantitative assay of coordinate gene expression in transgenic plants.This publication has 27 references indexed in Scilit:
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