Two Pairs of Recombination Signals Are Sufficient to Cause Immunoglobulin V-(D)-J Joining
- 20 November 1987
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 238 (4830), 1134-1138
- https://doi.org/10.1126/science.3120312
Abstract
The minimum sequence requirements for antigen receptor V-(D)-J joining were studied by constructing recombination-substrates containing synthetic recombination signals and introducing them into a recombination-competent pre-B cell line. Two sets of heptamer (CACTGTG) and nonamer (GGTTTTTGT) sequences were shown to be sufficient to cause the V-(D)-J joining, if the 12- and 23-base pair spacer rule is satisfied. A point mutation in the heptamer sequence, or a change in the combination of the two spacer lengths, drastically reduced the recombination.This publication has 32 references indexed in Scilit:
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