Detection of Microspotted Carcinoembryonic Antigen on a Glass Substrate by Scanning Electrochemical Microscopy
- 1 April 1996
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 68 (7), 1276-1278
- https://doi.org/10.1021/ac950824c
Abstract
Microspots of carbinoembryonic antigen (CEA) on glass substrates were characterized by scanning electrochemical microscopy (SECM). CEA was immobilized via a sandiwch method using horseradish peroxidase (HRP)-labeled anti-CEA. The reduction current of the oxidized form of ferrocenylmethanol generated by the HRP reaction was monitored to view SECM images. This method detects as low as ∼104 CEA molecules in a single 20-μm-radius spot.Keywords
This publication has 13 references indexed in Scilit:
- Imaging of immobilized antibody layers with scanning electrochemical microscopyAnalytical Chemistry, 1995
- Microscopic EquilibriaAnalytical Chemistry, 1995
- Separation-Free Sandwich Enzyme Immunoassays Using Microporous Gold Electrodes and Self-Assembled Monolayer/Immobilized Capture AntibodiesAnalytical Chemistry, 1994
- Advances in scanning electrochemical microscopy. Plenary lectureThe Analyst, 1994
- Small-volume voltammetric detection of 4-aminophenol with interdigitated array electrodes and its application to electrochemical enzyme immunoassayAnalytical Chemistry, 1993
- Automatic apparatus for heterogeneous enzyme immunoassays based on electrocatalytic detection of the enzyme and electrochemical regeneration of the solid phaseAnalytica Chimica Acta, 1993
- Chemical MicrosensorsScience, 1991
- Scanning Electrochemical MicroscopyAnalytical Chemistry, 1989
- Bioelectrochemical immunoassay for human chorionic gonadotrophin in serum using an electrode-immobilised capture antibodyBiosensors, 1986
- Enzyme immunosenserAnalytical Biochemistry, 1979