Differential effects of cholesterol and lanosterol on artificial membranes.
- 1 November 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (11), 4924-4926
- https://doi.org/10.1073/pnas.74.11.4924
Abstract
The effects of cholesterol, 4,4-dimethylcholesterol, and lanosterol (4,4'',14.alpha.-trimethyl-.DELTA.824-cholestadiene-3.beta.-ol) on some properties of lecithin vesicles were compared. Unlike cholesterol, lanosterol retards the exit of trapped glucose from phospholipid vesicles only slightly. The 13C NMR spectrum of cholesterol/lecithin vesicles shows no resonances attributable to the sterol. Several resonances attributable to quaternary C atoms or methyl groups are seen in the 13C NMR spectrum of lanosterol/lecithin vesicles, indicating that lanosterol is much less immobilized than cholesterol. Because the membrane behavior of 4,4-dimethylcholesterol is closely similar to that of cholesterol, the axial 14-.alpha.-methyl group is probably responsible for the lessened membrane immobilization of lanosterol. The results emphasize the importance of a planar sterol .alpha.-face for interaction with phospholipid acyl chains.This publication has 18 references indexed in Scilit:
- Isolation and partial characterization of a cholesterol-requiring mutant of Chinese hamster ovary cells.Proceedings of the National Academy of Sciences, 1977
- Structure in the polar head region of phospholipid bilayers: a phosphorus-31 {proton} nuclear Overhauser effect studyBiochemistry, 1976
- Roles of carbonyl oxygens at the bilayer interface in phospholipid–sterol interactionNature, 1976
- Headgroup conformation and lipid--cholesterol association in phosphatidylcholine vesicles: a 31P(1H) nuclear Overhauser effect study.Proceedings of the National Academy of Sciences, 1975
- Model of interaction of polar lipids, cholesterol, and proteins in biological membranesLipids, 1974
- Molecular Mechanism for the Interaction of Phospholipid with CholesterolNature New Biology, 1972
- The effect of sterol structure on the permeability of lipomes to glucose, glycerol and Rb+Biochimica et Biophysica Acta (BBA) - Biomembranes, 1972
- Correlation of In Vivo and In Vitro Phase Transitions of Membrane Lipids in Escherichia coliProceedings of the National Academy of Sciences, 1970
- The detection of oxidation in liposome preparationsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1970
- The Biological Synthesis of CholesterolScience, 1965