Assay of Phenobarbital: Adaptation of "EMIT" to the Centrifugal Analyzer

Abstract
We have adapted the centrifugal analyzer to the homo-geneous enzyme immunoassay ("EMIT"®) for phenobar-bital. The assay was modified to give greater range and sensitivity, and less reagent is needed. The transfer disc is prepared with totally automatic pipetting. Results are calculated with a micro-computer that provides a logit transformation of absorbance data. Precision and accuracy are excellent, and results correlate well with those by gas—liquid chromatography.