Silanophilic Interaction in the Retention Mechanism of Pharmaceutical Drugs on a Cyanopropyl Bonded Silica Column
- 1 April 1991
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 14 (8), 1561-1573
- https://doi.org/10.1080/01483919108049635
Abstract
The mechanism of retention of several pharmaceutical drugs with basic, neutral and acidic properties have been examined on a cyano bonded silica stationary phases (Ultrasphere cyano). Clenbuterol, bromhexine, terphenadine, pirenzepine, astemizol, betamethasone, acetaminophen, indomethacin, diclofenac and nimesulide were chosen for this study. Methanol-phosphate buffer mixtures with low water levels were used as mobile phases. Different pH values and ionic strengths were considered. Under experimental conditions, the chromatographic behavior of basic drugs is more complex than the behavior of acidic and neutral ones. The predominant silanophilic interactions observed for drugs of basic characteristics may become quite useful in optimizing selectivity.Keywords
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