A multimycotoxin TLC method is described for analysis of corn. Aflatoxins are extracted from the samples with acetonitrile-water, and NaHCO3 is added to separate the acidic ochratoxin from zearalenone and aflatoxin B1. After chloroform extraction, 1N NaOH is added to separate zearalenone and aflatoxin B1. The separated mycotoxins are spotted on TLC plates, which are then examined under UV light. The following recoveries (%) were obtained for corn samples: aflatoxin B1 71, ochratoxin A 87 and zearalenone 85. Limits of detection for the respective mycotoxins were 2, 40 and 200 ppb.