Inhibition of short-circuit current by triaminopyrimidine in isolated toad urinary bladder
- 30 April 1979
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 236 (5), C221-C224
- https://doi.org/10.1152/ajpcell.1979.236.5.c221
Abstract
The organic cation 2,4,6-triaminopyrimidine (TAP) produced inhibition of short-circuit current (SCC) when added to either the mucosal or serosal surface of the isolated urinary bladder of the toad. Fifty percent inhibition was produced by 10(-3) M TAP in the mucosal solution at pH 6.8 when the mucosal [Na+] was 113 mM. The actions of TAP resemble those produced by amiloride in several ways: a) inhibition of SCC by mucosal application is rapid; b) the mucosal inhibition is fully reversible; c) high concentrations in the serosal solutions produce irreversible inhibition; and d) the concentration required to produce 50% inhibition from the mucosal side is reduced when mucosal [Na+] is reduced. It is postulated that mucosal application of TAP and amiloride inhibit short-circuit current in high-resistance epithelia via action at a common locus.This publication has 7 references indexed in Scilit:
- Mode of action of amiloride in toad urinary bladderThe Journal of Membrane Biology, 1977
- Blockage of cation permeability across the tight junctions of gallbladder and other leaky epitheliaNature, 1974
- Amiloride and the sodium channelNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1974
- Effect of ouabain on sodium transport across hormone-stimulated toad bladder and skinPflügers Archiv - European Journal of Physiology, 1974
- Amiloride: a potent inhibitor of sodium transport across the toad bladderThe Journal of Physiology, 1968
- The mechanism of action of amipramizidePflügers Archiv - European Journal of Physiology, 1968
- The Coupling of the Short-Circuit Current to Metabolism in the Urinary Bladder of the ToadThe Journal of general physiology, 1963