Calorimetric Studies on Hydrolysis of Glucosides

Abstract

Calorimetric studies on the hydrolytic reactions of maltose and phenly [alpha]-maltoside catalyzed by gluc-amylase of Rhodo-spirillum Delemar have been made using a Dewar vessel type calorimeter. In order to obtain the adiabatic change of temperature in the system, a graphical adiabatic treatment method on a time-temperature curve has been devised. With this method it is unnecessary to attain the complete thermal equilibrium between the calorimeter and the surroundings and also to estimate the heat of stirring. The heats of hydrolysis of maltose and phenyl [alpha] -maltoside have been found to be [DELTA] H= [long dash]1100 cal/[image] and -1070 cal/[image] at 25[degree] C and [DELTA]h= -1140 cal/[image] and -1140 cal/[image] at 35 C, respectively.