Initiation by Yeast RNA Polymerase II at the Adenoviral Major Late Promoter in Vitro
- 3 November 1989
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 246 (4930), 661-664
- https://doi.org/10.1126/science.2510298
Abstract
Transcription of the yeast CYC1 promoter fused to a sequence lacking guanosine residues provided a rapid, sensitive assay of initiation by RNA polymerase II in yeast extracts. Initiation was enhanced by yeast and mammalian activator proteins. The adenoviral major late promoter fused to the G-minus sequence was transcribed in yeast extracts with an efficiency comparable to that observed in HeLa extracts, showing that promoters as well as transcription factors are functionally interchangeable across species. Initiation occurred at different sites, approximately 30 and 63 to 69 base pairs downstream of the TATA element of the adenoviral promoter in HeLa and yeast extracts, respectively, distances characteristic of initiation in the two systems in vivo. A component of the transcription system and not the promoter sequence determines the distance to the initiation site.This publication has 12 references indexed in Scilit:
- Nucleosome loss activates yeast downstream promoters in vivoCell, 1988
- GAL4-VP16 is an unusually potent transcriptional activatorNature, 1988
- Function of a yeast TATA element-binding protein in a mammalian transcription systemNature, 1988
- A yeast activity can substitute for the HeLa cell TATA box factorNature, 1988
- GAL4 activates gene expression in mammalian cellsCell, 1988
- The yeast UASG is a transcriptional enhancer in human hela cells in the presence of the GAL4 trans-activatorCell, 1988
- The control of transcription in Saccharomyces cerevisiaeBioEssays, 1987
- Tissue-specific in vitro transcription from the mouse albumin promoterCell, 1986
- Transcription initiation of the Saccharomyces cerevisiae iso-1-cytochrome c geneJournal of Molecular Biology, 1986
- In vivo sequence requirements of the SV40 early promoter regionNature, 1981