Prolific plant regeneration from protoplast-derived tissues of Lotus corniculatus L. (birdsfoot trefoil)

Abstract
Protoplasts isolated enzymatically from seedling roots, hypocotyls and cotyledons of Lotus corniculatus L. produced callus which underwent prolific shoot regeneration. The rapidity and ease of recovering plants from protoplast-derived tissues makes this forage legume an attractive experimental system for genetic manipulation.