The influence of chloride and other univalent inorganic anions on the visible-absorption spectrum of aspartate aminotransferase
- 1 December 1968
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 110 (3), 471-473
- https://doi.org/10.1042/bj1100471
Abstract
1. The influence of Cl−, Br−, NO3− and F− ions on the visible-absorption spectrum of deionized aspartate aminotransferase was investigated. 2. Except for F−, these anions caused an increase of the extinction at 430mμ with a concomitant decrease of that at 362mμ. 3. The affinity constants for Cl− and NO3− ions were calculated by a procedure based on the assumption that the anion stabilizes the protonated form of the enzyme chromophore (λmax. 430mμ). 4. The true pK of the chromophore of the enzyme was found to be 5·25.Keywords
This publication has 4 references indexed in Scilit:
- Isolation and Characterization of Multiple Forms of Glutamate-Aspartate Aminotransferase from Pig HeartJournal of Biological Chemistry, 1967
- Purification and general properties of aspartate aminotransferase of ox heartBiochemical Journal, 1966
- [Purification of aspartate aminotransferase from the bovine heart].1965
- GLUTAMIC ASPARTIC TRANSAMINASE .1. ASSAY, PURIFICATION, AND GENERAL PROPERTIES1959