Mitochondrial DNA variability in natural populations of Hawaiian Drosophila. I. Methods and levels of variability in D. silvestris and D. heteroneura populations
- 1 February 1986
- journal article
- research article
- Published by Springer Nature in Heredity
- Vol. 56 (1), 75-85
- https://doi.org/10.1038/hdy.1986.11
Abstract
We describe techniques by which mitochondrial DNA (mtDNA) restriction site information can be obtained for up to 16 different restriction endonucleases on individual Hawaiian Drosophila, in particular D. silvestris and D. heteroneura. We have constructed mtDNA restriction site maps for a total of forty-eight wild caught genomes of both species, from seven major collecting sites on the island of Hawaii. Levels of variability are, in general, high in D. silvestris (p of Ewens et al., 1981 = 00486) and lower in D. heteroneura (p=00327). Measures of population subdivision using Nei's Gst indicate that about 50–60 per cent of the observed variability is due to interdemic subdivision. In accordance, populations within a species show a much lower level of variability, however some populations harbour individuals that are very divergent from the rest of their conspecifics at the same locality. We review two possible mechanisms that could explain the presence of these divergent individuals, hybridisation and the effects of stochastic branching processes.Keywords
This publication has 43 references indexed in Scilit:
- Drosophila melanogaster mitochondrial DNA, a novel organization and genetic codeNature, 1983
- Sexual Selection and Direction of Evolution in the Biosystematics of Hawaiian DrosophilidaeAnnual Review of Entomology, 1983
- Rate of turnover of structural variants in the rDNA gene family of Drosophila melanogasterNature, 1982
- Maternal inheritance of human mitochondrial DNA.Proceedings of the National Academy of Sciences, 1980
- Inter- and intraspecific variation in restriction maps of Drosophila mitochondrial DNAsNature, 1979
- Mathematical model for studying genetic variation in terms of restriction endonucleases.Proceedings of the National Academy of Sciences, 1979
- Mitochondrial DNA Analyses and the Origin and Relative Age of Parthenogenetic Lizards (Genus Cnemidophorus )Science, 1979
- Natural Hybridization between a Sympatric Pair of Hawaiian DrosophilaThe American Naturalist, 1977
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Drosophila of Hawaii: Systematics and Ecological GeneticsAnnual Review of Ecology and Systematics, 1976