The six-minute walk test (6MWT) is an inexpensive, quick and safe tool to evaluate the functional capacity of patients with heart failure and chronic obstructive pulmonary disease. The aim of this study was to determine the reproducibility of the 6MWT in overweight and obese individuals. We thus undertook a prospective repeated-measure validity study taking place in our academic weight management outpatient clinic. The 6MWT was conducted twice the same day in 21 overweight or obese adult subjects (15 females and 6 males). Repeatability of walking distance was the primary outcome. Anthropometric measures, blood pressure and heart rate were also recorded. Participant's mean BMI was 37.2±9.8 kg/m2 (range: 27.0–62.3 kg/m2). Walking distance in the morning (mean=452±90 m) and in the afternoon (mean=458±97 m) were highly correlated (r=0.948; 95% Confidence Interval 0.877–0.978; p<0.001). Walking distance was negatively correlated with BMI (r=−0.47, p=0.03), waist circumference (r=−0.43, p=0.05) and pre-test heart rate (r=−0.54, p=0.01). Our findings indicate that the 6MWT is highly reproducible in obese subjects and could thus be used as a fitness indicator in clinical studies and clinical care in this population.