Gel Permeation Chromatography: The Effect of Sample Size on Efficiency

Abstract

Various reported studies of the effect of sample size on efficiency in gel permeation chromatography are not in complete agreement. In particular there is conflicting experimental evidence as to the existence of a lower limit in sample size below which no further increase in efficiency is observed. This study reports efficiency studies in terms of plate number and resolution for polystyrene in chloroform for sample sizes less than those commonly studied. The results support the existence of such a lower limit on sample size.