Demonstration of cyclic AMP in bone cells by immuno-histochemical methods
- 1 December 1975
- journal article
- research article
- Published by Springer Nature in Calcified Tissue International
- Vol. 19 (1), 305-315
- https://doi.org/10.1007/bf02564013
Abstract
By utilizing immunohistochemical procedures it was possible to locate bone cells that contained adenosine 3′5′-monophosphate (cyclic AMP) in frozen, undecalcified cat jaw sections. An immunoglobulin-enzyme bridge method was found to be superior to other techniques, including immunofluorescence. Differences in staining pattern were found between bone cells of kittens and young adult cats, indicating different metabolic levels related to cellular maturity, and different intracellular compartmentalization of cyclic AMP in the two age groups.This publication has 14 references indexed in Scilit:
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