Interactions of rat α-foetoprotein with bilirubin
- 31 May 1979
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 179 (3), 705-707
- https://doi.org/10.1042/bj1790705
Abstract
The binding of bilirubin to rat-alpha-foetoprotein has been measured by changes in the absorption spectrum and by the appearance of two intense circular-dichroism bands. Furthermore binding of this bile pigment has been demonstrated by a decrease of its enzymic oxidation rate and by competition experiments with bovine serum albumin-agarose. The binding parameters have been determined as follows: n = 1.0 mol bound/mol of protein and Ka = 2.9 × 10(6) M-1. Competition of oestradiol for the bilirubin-binding site has not been established.This publication has 16 references indexed in Scilit:
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