An Electrophoretic Characterization of the Glucocorticoid Response of the Fu5‐5 Rat Hepatoma Cell Line

Abstract

The response of liver-derived cells to glucocorticoid treatment was characterized using the rat hepatoma, Fu5-5. Two-dimensional electrophoresis was used to examine changes in the synthetic rats of cytosol proteins following glucocorticoid administration by using [35S]methionine. Changes occurred in the incorporation of 32P by several cytosol proteins after hormone treatment. One of these changes occurs within 1 h of hormone treatment. No changes in the nuclear protein content of Fu6-5 cells were found after glucorticoid treatment. The glucocorticoid-regulated changes in cytosol protein synthesis in Fu5-5 cells were compared with those of the H35 rat hepatoma, monolayer cultures of rat hepatocytes and the response of rat liver in vivo. Although nearly all of the 18 hormonally responsive proteins appeared to be present in all of the cell types, only 3 were responsive to glucocorticoid in more than 1 system. Moreover, the response patterns were often reversed in different cell types, the same protein being induced in one cell type and repressed in another.