Analysis of the Serum IgE Levels in Nonimmunized Rats of Various Strains by a Radioimmunoassay
- 1 March 1979
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 9 (3), 217-228
- https://doi.org/10.1111/j.1365-3083.1979.tb02725.x
Abstract
An assay, the paper radioimmunosorbent test (PRIST) for the measurement of total serum IgE in the rat, that allows the measurement of IgE down to a level of 0.25 ng/ml, is described. With this assay it has been demonstrated that significant differences exist in normal serum IgE levels in non‐immunized rats. These differences are strain‐dependent, and strains may be classified as to low (300 ng/ml) serum levels. The serum IgE level is not sex‐dependent. Environmental factors have a pronounced influence, optimal housing conditions resulting in low and stable levels while suboptimal conditions result in high levels with great fluctuations. Hybridization experiments suggest a multigenic control, with low IgE strains having a dominating suppressor effect.This publication has 13 references indexed in Scilit:
- A Radioimmunoassay for Evaluation of the IgE and IgG Antibody Responses in the RatScandinavian Journal of Immunology, 1979
- The measurement of total serum IgE levels in ratsJournal of Immunological Methods, 1977
- IgE formation in the rat following infection with Nippostrongylus brasiliensisCellular Immunology, 1976
- Function and Structure of Immunoglobulin E (IgE)Published by Springer Nature ,1976
- Elevation of total serum IgE in rats following helminth parasite infectionNature, 1974
- Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, Ige and IgG1, IgG2a, IgG2b, IgG2cEuropean Journal of Immunology, 1974
- A new and simple radioimmunoassay method for the determination of IgEMolecular Immunology, 1972
- Structure and Function of Human Immunoglobulin EAdvances in Immunology, 1971
- Chemical Coupling of Proteins to AgaroseNature, 1967
- Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific ActivityNature, 1962