Influence of Follicular Maturation upon the Effect of Ovine Follicle Stimulating Hormone and Luteinizing Hormone upon Cyclic AMP Accumulation by Isolated Porcine Granulosa Cells
- 1 April 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 20 (3), 473-482
- https://doi.org/10.1095/biolreprod20.3.473
Abstract
The ability of ovine follicle stimulating hormone (oFSH) and ovine luteinizing hormone (oLH) to stimulate cyclic[c]AMP accumulation was examined in granulosa cells harvested from small (1-2 mm), medium (3-5 mm) and large (6-12 mm) porcine follicles. The oFSH was pretreated with antiserum to LH to neutralize contaminating LH. The cells were incubated in Eagle''s medium plus 1% BSA [bovine serum albumin] for varying periods of time ranging from 1-60 min. cAMP accumulation in the cells and in the incubation medium was measured by a protein binding assay. In cells harvested from small follicles, 10 .mu.g of oFSH produced a significant stimulation in intracellular cAMP (791 .+-. 121%; n = 6) after 1 min of incubation and produced a maximal 23-fold (2340 .+-. 34.7%; n = 6) stimulation of intracellular cAMP after 5 min of incubation. When cells harvested from small, medium and large follicles were incubated for 15 min with 10 .mu.g oFSH, a 1802 .+-. 109% (n = 11), 520 .+-. 97% (n = 11) and a 151 .+-. 20% (n = 8) stimulation of intracellular cAMP accumulation, respectively, was observed. When FSH was present throughout an incubation period of 60 min, there was an initial stimulation of intracellular cAMP accumulation followed by a decline in intracellular cAMP between 30 and 60 min of incubation in the case of cells from all 3 types of follicles. After 30 and 60 min of incubation with cells obtained from small and medium follicles, 10 .mu.g of oFSH resulted in an increased accumulation of cAMP in the incubation medium. The responsiveness of granulosa cells to oLH was dissimilar to the response to FSH. Addition of 1.0 .mu.g oLH for a 15 min incubation period led to a 153 .+-. 19% (n = 9), 410 .+-. 65% (n = 11) and a 1139 .+-. 90% (n = 8) stimulation of intracellular cAMP accumulation in granulosa cells from small, medium and large follicles, respectively. During 30 and 60 min incubation periods, LH stimulation of cells obtained from medium and large follicles resulted in accumulation of cAMP in the incubation medium.This publication has 10 references indexed in Scilit:
- Effect of a Phosphodiesterase Inhibitor, 3-lsobutyl 1-methylxanthine, upon the Stimulatory Effect of Human Follicle-Stimulating Hormone and Human Luteinizing Hormone upon Cyclic Adenosine 3':5'-Monophosphate Accumulation by Porcine Granulosa CellsExperimental Biology and Medicine, 1978
- Effects of Follicle-Stimulating Hormone and Estradiol upon Progesterone Secretion by Porcine Granulosa Cells in Tissue Culture*Endocrinology, 1978
- Binding of LH and FSH to porcine granulosa cells during follicular maturationReproduction, 1977
- Mechanism of action of luteinizing hormone and follicle-stimulating hormone on the ovary in vitroMetabolism, 1977
- The Porcine Ovarian Follicle: I. Selected Chemical Analysis of Follicular Fluid at Different Developmental Stages1Biology of Reproduction, 1976
- The Porcine Ovarian Follicle: III. Development of Chorionic Gonadotropin Receptors Associated with Increase in Adenyl Cyclase Activity During Follicle MaturationEndocrinology, 1976
- Adenylyl Cyclase Activities in Ovarian Tissues. IV. Gonadotrophin-Induced Desensitization of the Luteal Adenylyl Cyclase Throughout Pregnancy and Pseudopregnancy in the Rabbit and the RatEndocrinology, 1976
- Adenylyl Cyclase Activities in Ovarian Tissues. II. Regulation of Responsiveness to LH, FSH, and PGE1in the RabbitEndocrinology, 1976
- Hormone-stimulated desensitization of hormone-dependent adenylyl cyclase. Dual action of luteninizing hormone on pig graafian follicle membranes.Journal of Biological Chemistry, 1976
- The Stimulatory Effect of Luteinizing Hormone on Adenosine 3‘,5‘-Monophosphate Accumulation in Corpus Luteum SlicesJournal of Biological Chemistry, 1966