Specific transcription of a 15-kilodalton zene gene in HeLa cell extracts
- 1 January 1986
- journal article
- research article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 7 (1), 71-79
- https://doi.org/10.1007/bf00020133
Abstract
A maize genomic clone containing a 15 kilodalton zein gene was used as a template in an in vitro transcription system for HeLa cells. A runoff assay indicated transcription was initiating 5′ to the map position of the open reading frame for the protein. Fine-structure mapping of RNAs synthesized in vitro showed two transcription start sites separated by 24 bases. One start site is 27 bases downstream of a consensus TATA sequence; the other is 30 bases downstream of a TATG sequences. The initiation sites for RNA synthesized in vitro map to the same region of the genomic clone as zein RNA isolated from developing maize kernels.This publication has 39 references indexed in Scilit:
- Zein gene organization in maize and related grassesJournal of Molecular Evolution, 1984
- A zein gene of maize is transcribed from two widely separated promoter regionsCell, 1983
- Human β-globin promoter and coding sequences transcribed by RNA polymerase IIICell, 1983
- Site-specific base substitution and deletion mutations that enhance or suppress transcription of the SV40 major late RNACell, 1982
- Transcription of a gene for human U1 small nuclear RNACell, 1982
- Complex organization of zein genes in maizeGene, 1981
- DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.Proceedings of the National Academy of Sciences, 1980
- Isolation of undegraded polysomes from radish cotyledons: Use of proteinase K and cycloheximidePhytochemistry, 1980
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- Genetic regulation of storage protein content in maize endospermBiochemical Genetics, 1976