The action of staphylococcal toxins on isolated rabbit intestine

Abstract

Staphylococcal α- and β-toxins inhibited the spontaneous activity of isolated rabbit intestine, the former leading to an increase in tone and the latter to a decrease. The γ-toxin and δ-haemolysin had no effect in concentrations likely to be met in extracts of cultures, nor did δ-lysin enhance the action of β-toxin. Culture extracts in which the haemolytic toxins had been neutralized by antiserum sometimes produced an increase in tone or amplitude or both, which was moderate in degree but rapid in onset. This stimulation was more common with strains isolated from food-poisoning outbreaks than from infective lesions. It was occasionally observed with coagulase-negative strains and was therefore considered to be unrelated to enterotoxin. No effect on intestine was observed which could be considered specific for food-poisoning strains.We are indebted to Dr R. E. O. Williams of the Central Public Health Laboratory, Colindale, for providing food-poisoning strains of staphylococci, to Dr H. W. Smith for strain CN, and the Wellcome Research Laboratories for gifts of sera.