Oxidation of Palmitic Acid-1-C14 by Tissues of Carbohydrate and Fat Diet-Adapted Rats

Abstract

Groups of adult male rats were adapted to isocaloric high fat and high carbohydrate diets for 4–6 weeks. Liver slices and segments of diaphragm were incubated in a medium to which an emulsion containing palmitic acid-1-C¹⁴ had been added. Respiratory CO₂ was collected, precipitated as BaCO₃, and the specific activity estimated. Liver slices of fat diet-adapted rats oxidized more of the added palmitic acid than did those of carbohydrate-fed controls. Diaphragms of the former group also oxidized more added palmitic acid but abstracted less glucose from the medium than did those of the latter.