The bacteriochlorophyll biosynthesis gene, bchM, of Rhodobacter sphaeroides encodes S‐adenosyl‐L‐methionine: Mg protoporphyrin IX methyltransferase
- 26 September 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 352 (2), 127-130
- https://doi.org/10.1016/0014-5793(94)00934-1
Abstract
The bchM gene of Rhodobacter sphaeroides has been sequenced and then overexpressed in E. coli producing a protein of M r approximately 27,500. Cell-free extracts of the transformed E. coli strain are able to methylate added Mg protoporphyrin, resulting in the formation of Mg protoporphyrin monomethyl ester. The identity of this product was verified by HPLC. The bchM gene product is therefore assigned to the methyltransferase step in bacteriochlorophyll biosynthesis.Keywords
This publication has 19 references indexed in Scilit:
- Gene expression and control of enzymes for synthesis of magnesium protoporphyrin monomethyl ester in Rhodobacter sphaeroides.Biochemical Society Transactions, 1993
- Isolation and characterization of Escherichia coli mutants affected in aerobic respiration: the cloning and nucleotide sequence of ubiG: Identification of an S-adenosylmethionine-binding motif in protein, RNA, and small-molecule methyltransferasesJournal of General Microbiology, 1992
- Nucleotide Sequence of S-Adenosyl-l-Methionine: Magnesium Protoporphyrin Methyltransferase from Rhodobacter capsulatusPlant Physiology, 1992
- Gentic evidence for superoperonal organization of genes for photosynthesis pigments and pigment-binding proteins in Rhodobacter capsulatusMolecular Genetics and Genomics, 1989
- Construction of a physical map of the 45 kb photosynthetic gene cluster of Rhodobacter sphaeroidesArchiv für Mikrobiologie, 1989
- Vectors for selective expression of cloned DNAs by T7 RNA polymeraseGene, 1987
- Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genesJournal of Molecular Biology, 1986
- Genetic-physical mapping of a photosynthetic gene cluster from R. capsulataCell, 1984
- Magnesium protoporphyrin chelatase activity in Rhodopseudomonas spheroides. Studies with whole cellsBiochemical Journal, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970