Hemocyanins in Spiders, XVIII. Complete Amino-Acid Sequence of SubunitefromEurypelma californicumHemocyanin
- 1 January 1983
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 364 (2), 1357-1382
- https://doi.org/10.1515/bchm2.1983.364.2.1357
Abstract
The complete amino-acid sequence of subunit e of the hemocyanin from the tarantula, E. californicum, was determined. By limited proteolysis with chymotrypsin, 2 large fragments (e-CHn 29 and e-CHn 42) were obtained. The large peptides were further cleaved with cyanogen bromide, trypsin (with and without prior blocking of lysine residues), chymotrypsin, Staphylococcus aureus proteinase, Astacus fluviatilis proteinase, or 25% formic acid. The complete chain comprises 621 residues. A remarkable feature of the sequence is a hexapeptide -His-His-Trp-His-Trp-His- which is believed to take part in the binding of Cu.This publication has 19 references indexed in Scilit:
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