Disappearance of the Lymphoid System in Bcl-2 Homozygous Mutant Chimeric Mice
- 17 September 1993
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 261 (5128), 1584-1588
- https://doi.org/10.1126/science.8372353
Abstract
The bcl-2 proto-oncogene can prevent the death of many cell types. Mice were generated that were chimeric for the homozygous inactivation of bcl-2. Lymphocytes without Bcl-2 differentiated into phenotypically mature cells. However, in vitro, the mature T cells that lacked Bcl-2 had shorter life-spans and increased sensitivity to glucocorticoids and gamma-irradiation. In contrast, stimulation of CD3 inhibited the death of these cells. T and B cells with no Bcl-2 disappeared from the bone marrow, thymus, and periphery by 4 weeks of age. Thus, Bcl-2 was dispensable for lymphocyte maturation, but was required for a stable immune system after birth.Keywords
This publication has 31 references indexed in Scilit:
- p53 is required for radiation-induced apoptosis in mouse thymocytesNature, 1993
- Elimination of self-reactive B lymphocytes proceeds in two stages: Arrested development and cell deathCell, 1993
- bcl-2 inhibits multiple forms of apoptosis but not negative selection in thymocytesCell, 1991
- Mature murine B and T cells transferred to SCID mice can survive indefinitely and many maintain a virgin phenotype.The Journal of Experimental Medicine, 1991
- Kinetics and efficacy of positive selection in the thymus of normal and T cell receptor transgenic miceCell, 1991
- Neonatal lethality and lymphopenia in mice with a homozygous disruption of the c-abl proto-oncogeneCell, 1991
- Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell deathNature, 1990
- Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocationCell, 1986
- Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around Jh on chromosome 14 and near a transcriptional unit on 18Cell, 1985
- Cloning of the Chromosome Breakpoint of Neoplastic B Cells with the t(14;18) Chromosome TranslocationScience, 1984