A Novel Chloride‐Dependent L‐[3H]Glutamate Binding Site in Astrocyte Membranes

Abstract

Membrane fractions prepared from astrocytes grown in culture exhibit a specific binding site for L-[3H]glutamate that is Cl- -dependent and Na+ -independent. The binding site is a single saturable site with with a KD of about 0.5 .mu.M, is inhibited by L-aspartate, L-cysteate, and quisqualate, and is insensitive to kainate, N-methyl-D-aspartate, .alpha.-amino-3-hydroxy-5-methyl-4-isoxazole propionate, and 2-amino-4-phosphonobutyrate. The pharmacological characteristics of the binding site indicate that it is distinct from any site previously described in synaptic membrane preparations. Comparisons of ionic requirements, ligand specificity, and inhibitor sensitivities, however, suggest the described binding is the first step in a Cl- -dependent high-affinity glutamate uptake system. Such binding studies provide a useful model system in which to investigate the close association between excitatory amino acids, astrocytes, the termination of glutamate''s excitatory action by high-affinity uptake, and the excitotoxic action of acidic amino acids in membranes of a single cell type.