Expression of the cloned endo-1,3-1,4-?-glucanase gene of Bacillus subtilis in Saccharomyces cerevisiae
- 1 August 1984
- journal article
- Published by Springer Nature in Current Genetics
- Vol. 8 (6), 471-475
- https://doi.org/10.1007/bf00433914
Abstract
A cloned endo-1,3-1,4-β-glucanase gene from the Gram-positive bacterium B. subtilis has been located by deletion analysis on a 1.4 kb PvuI-ClaI DNA fragment. This gene has been sub-cloned in the yeast LEU2 vector pJDB207 to produce a hybrid plasmid designated pEHB9. pEHB9 has been transformed to S. cerevisiae and shown to direct the synthesis of an endo-1,3-1,4-β-glucanase in yeast. The β-glucanase activity was low and could only be detected in crude cell extracts of yeast harbouring pEHB9.Keywords
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