Chemical Composition of the Protoplast Membrane of Bacillus megaterium
- 1 April 1966
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 59 (4), 374-381
- https://doi.org/10.1093/oxfordjournals.jbchem.a128312
Abstract
The chemical composition of the protoplast membrane of B. megaterium NRRL B939 was studied. The protoplast membrane prepared in the presence of Mg ion accounted for 30% of the dry weight of intact cells. Its chemical composition/g membrane fraction was: protein 450-550 mg, ribonucleic-acid (RNA) 70-100 mg, deoxyribonucleic-acid (DNA) < 10 mg, lipid 40-60 mg, carbohydrate < 10 mg and poly-[beta]-hydroxybutyrate 350-500 mg. The phospholipids and neutral lipids accounted for 67 and 33% of total lipids, respectively. The dominant components in the lipid fraction were phosphatidylethanolamine, phosphatidylglycerol, cardiolipin, diglyceride, monoglyceride and free fatty-acids. The chemical composition of other strains of B. megaterium was presented.This publication has 16 references indexed in Scilit:
- ELECTRON MICROSCOPY OF BACILLUS MEGATERIUM UNDERGOING ISOLATION OF ITS NUCLEAR BODIESJournal of Bacteriology, 1964
- Protein synthesis by polyribosomes on protoplast membranes of B. megateriumJournal of Molecular Biology, 1963
- Cellular components of Bacillus megaterium and their role in protein biosynthesisBiochemical Journal, 1961
- ASSAY OF POLY-β-HYDROXYBUTYRIC ACIDJournal of Bacteriology, 1961
- Separation of lipid classes by chromatography on florisilJournal of Lipid Research, 1961
- A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samplesBiochemical Journal, 1960
- The chemical composition of the protoplast membrane of Micrococcus lysodeikticusBiochimica et Biophysica Acta, 1958
- A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUESJournal of Biological Chemistry, 1957
- DETERMINATION OF PENTOSE IN THE PRESENCE OF LARGE QUANTITIES OF GLUCOSE1946
- The estimation of phosphorusBiochemical Journal, 1940