The terminal oxidases of Paracoccus denitrificans
- 1 July 1994
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 13 (2), 183-196
- https://doi.org/10.1111/j.1365-2958.1994.tb00414.x
Abstract
Three distinct types of terminal oxidases participate in the aerobic respiratory pathways of Paracoccus denitrificans. Two alternative genes encoding sub unit I of the aa3‐type cytochrome c oxidase have been isolated before, namely ctaDI and ctaDII. Each of these genes can be expressed separately to complement a double mutant (ActaDI, ActaDII), indicating that they are isoforms of subunit I of the aa3‐type oxidase. The genomic locus of a quinol oxidase has been isolated: cyoABC. Thisprotohaem‐containing oxidase, called cytochrome bb3, is the oniy quinoi oxidase expressed under the conditions used, in a triple oxidase mutant (ActaDI, ActaDII, cyoB::KmR) an alternative cyto‐chrome c oxidase has been characterized; this cbb3‐type oxidase has been partially purified. Both cytochrome aa3 and cytochrome bb3 are redox‐driven proton pumps. The proton‐pumping capacity of cytochrome cbb3 has been analysed; arguments for and against the active transport of protons by this novel oxidase complex are discussed.Keywords
This publication has 71 references indexed in Scilit:
- The CuAsite of the caa3‐type oxidase of Bacillus subtilis is a mixed‐valence binuclear copper centreFEBS Letters, 1994
- Heme O biosynthesis in Escherichia coli: The cyoe gene in the cytochrome BO operon encodes a protoheme IX farnesyltransferaseBiochemical and Biophysical Research Communications, 1992
- Oxidation of methylamine by a Paracoccus denitrificans mutant impaired in the synthesis of the bc1 complex and the aa3‐type oxidase Evidence for the existence of an alternative cytochrome c oxidase in this bacteriumFEBS Letters, 1992
- Properties of the two terminal oxidases of Escherichia coliBiochemistry, 1991
- Are there isoenzymes of cytochrome c oxidase in Paracoccus denitrificans?FEBS Letters, 1990
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.Proceedings of the National Academy of Sciences, 1980
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980
- Induction by cyanide of cytochrome d in the plasma membrane of paracoccus denitrificansFEBS Letters, 1979
- The variable cytochrome content of Paracoccus Denitrificans grown aerobically under different conditionsFEBS Letters, 1978