Membrane phase transitions are responsible for imbibitional damage in dry pollen
- 1 January 1989
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 86 (2), 520-523
- https://doi.org/10.1073/pnas.86.2.520
Abstract
We have found that the most probable cause of the leakage seen when dry cells or organisms such as seeds, pollen, or yeast cells are plunged into water is a gel to liquid crystalline phase transition in membrane phospholipids accompanying rehydration. By using Fourier transform infrared spectroscopy we have recorded infrared spectra of CH2 stretching vibrations in dry and partially hydrated intact pollen grains of Typha latifolia. The vibrational frequency changes abruptly as phospholipids pass through the gel to liquid crystalline phase transition. Below the apparent transition, viable pollen shows low germination and high leakage when placed in water, but above the transition germination increases and leakage decreases. The apparent transition temperature falls with increasing water content, much as in pure phospholipids. By using this phenomenon, it was possible to construct a hydration-dependent phase diagram for the intact pollen. This phase diagram has immediate applications since it has high predictive value for the viability of the pollen when it is placed in water.This publication has 15 references indexed in Scilit:
- A crystalline lipid phase in a dry biological system: evidence from X-ray diffraction analysis of Typha latifolia pollenBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1987
- Imbibitional Chilling Injury in PollenPlant Physiology, 1984
- Membrane Isolation Alters the Gel to Liquid Crystal Transition of Acholeplasma laidlawii BScience, 1983
- Phosphatidylsulfocholine bilayersBiochimica et Biophysica Acta (BBA) - Biomembranes, 1982
- Effect of Dehydration on Leakage and Membrane Structure in Lotus corniculatus L. SeedsPlant Physiology, 1980
- Temperature Effects on Soybean Imbibition and LeakagePlant Physiology, 1980
- Effects of in vivo single and multiple isoproterenol injections on subsequently explanted submandibular glandsCells Tissues Organs, 1979
- Chilling Stress to Soybeans during ImhibitionPlant Physiology, 1978
- The effect of chain length and lipid phase transitions on the selective permeability properties of liposomesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1975
- Phase transitions in the yeast cell membrane the influence of temperature on the reconstitution of active dry yeastBiochimica et Biophysica Acta (BBA) - Biomembranes, 1974