Inhibition of Platelet Thromboxane Formation and Phosphoinositides Breakdown by Diisoeugenol
- 1 January 1994
- journal article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 46 (1), 54-57
- https://doi.org/10.1111/j.2042-7158.1994.tb03720.x
Abstract
Diisoeugenol inhibited the platelet aggregation and ATP release of rabbit platelets caused by ADP, arachidonic acid, platelet-activating factor (PAF), collagen and thrombin. Prolongation of the incubation time of platelets with diisoeugenol did not cause further inhibition and the aggregability of platelets could not be restored after washing. In human platelet-rich plasma, diisoeugenol inhibited the biphasic aggregation and ATP release induced by adrenaline and ADP in a concentration-dependent manner. Thromboxane B2 formation caused by arachidonic acid, collagen and thrombin was markedly inhibited by diisoeugenol in a concentration-dependent manner. Diisoeugenol also inhibited the formation of inositol monophosphate caused by collagen, PAF and thrombin. The cAMP level of washed platelets was not changed by diisoeugenol. It is concluded that the antiplatelet effect of diisoeugenol is due to the inhibition of thromboxane formation and phosphoinositides breakdown.Keywords
This publication has 14 references indexed in Scilit:
- Eugenol and Analogues as Antiplatelet CompoundsPlanta Medica, 1986
- The effects of lithium on platelet phosphoinositide metabolismBiochemical Journal, 1986
- Phosphoinositide turnover provides a link in stimulus—response couplingTrends in Biochemical Sciences, 1985
- Inositol trisphosphate and diacylglycerol as second messengersBiochemical Journal, 1984
- Platelet-activating factor stimulates phosphatidylinositol turnover in human plateletsBiochemical Journal, 1983
- Prostaglandins: Specific inhibition of platelet adhesion to collagen and relationship with cAMP levelProstaglandins, 1982
- Phospholipid Metabolism in Stimulated Human PlateletsJCI Insight, 1980
- Studies on the Photodimerization of IsoeugenolJournal of the Chinese Chemical Society, 1978
- [1] Purification and properties of firefly luciferasePublished by Elsevier ,1978
- Thromboxanes: a new group of biologically active compounds derived from prostaglandin endoperoxides.Proceedings of the National Academy of Sciences, 1975