Preparation Of Protein-Steroid Conjugates And Antisera Against 0Estradi0L-17β
Open Access
- 1 June 1973
- journal article
- research article
- Published by Springer Nature in Acta Veterinaria Scandinavica
- Vol. 14 (2), 278-291
- https://doi.org/10.1186/bf03547446
Abstract
A conjugate of oestradiol-17β and bovine serum albumin (BSA) has been prepared by the mixed anhydride method. The conjugate was characterized by u.v.-analyses and immunoelectrophoresis, and the number of moles of oestradiol-17β per mole of BSA was found to be 25. The conjugate was used for immunization of two sheep and one rabbit in order to elict antisera against oestradiol-17β. Antibodies could be detected in all three animals after 5–16 weeks depending on the route of immunization. The antiserum from one sheep could be used in a radioimmunoassay system for oestradiol-17β in a dilution of 1:5000 and was found to cross-react with oestrone and oestradiol-17α to a lesser extent than another antiserum against oestradiol-17β.Keywords
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