Alloxan Diabetes and Insulin Effects on Amino Acid Incorporating Activity of Rat Liver Microsomes.

Abstract

Liver microsomes from severely diabetic rats incorporated leucine-1-C14 into protein at a lower rate than microsomes from diabetic control rats maintained on insulin. The former also had a lower phos-pholipid-P and a higher ribonucleic acid content per unit of protein-N. Microsomes from normal rats injected with insulin incorporated amino acids at a higher rate. These changes occurred specifically in microsomes; no difference was found in the ability of soluble fractions to activate microsomal incorporation. Insulin was without effect upon the microsomal system in vitro.